DNA

Part:BBa_K1510114:Design

Designed by: Kao Jung Chang   Group: iGEM14_NYMU-Taipei   (2014-10-07)

C16 is a signal peptide that binds to the surface of Streptococcus Mutants.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We truncate mature CSP(21 amio acid) into smaller peptide CSPC16 .(16 amio acid) to avoid interaction with our modified phage. Fluorescently labeled CSPC16 bound to UA159 and the comD mutant in a similar manner with CSP, indicating that the specific binding ability of CSPC16 to S. mutans is independent of the presence of ComD.



Source

We derive our C16 peptide from the genome of Streptococcus Mutants UA159.

References

Quorum sensing and biofilm formation by streptococcus mutans(2008)Landes bioscience,Dilani senaadheera and Dannis G.Cvitkovitch